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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Chemical-defined medium supporting the expansion of human mesenchymal stem cells

Fig. 2

Full chemical-defined medium development. a Basal medium comparison. Human MSCs were plated onto p12 plates at 2 × 104 cells per well and the cell number was counted 5 days later. bd Growth factor and chemical screening. Human MSCs were plated onto p12 plates at 2 × 104 cells per well and the cell number was counted 5 days later. e Cell doubling time analysis for the human MSCs cultured with the FBS, 5% PL, and NBVbe medium. The cells were continuously cultured for 5 passages. FBS. fetal bovine serum; NB, the N2B27-VA medium with the BSA (bovine serum albumin) replaced by the recombinant human albumin; PL, platelet lysate; BIO, 6-bromoindirubin-3′-oxime; OSM, oncostatin M; Vc, vitamin C; PDGF, platelet-derived growth factor; bFGF, basic fibroblast growth factor; HGF, hepatocyte growth factor; EGF, epidermal growth factor; IGF, insulin-like growth factor 1; BDNF, brain-derived neurotrophic factor; NGF, nerve growth factor; VEGFA, vascular endothelial growth factor A; TGFb2, transforming growth factor beta 2; 11F, Vc + PDGF-AA + PDGF-AB + bFGF + HGF + EGF + IGF + BDNF + NGF + VEGFA + TGFb2; 4F, Vc + PDGF-AB + bFGF + EGF; 3F, Vc + bFGF + EGF. An asterisk indicates P < 0.05; N.S. indicates non-significant

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