Fig. 3

Characterization of human MSCs cultured with the NBVbe medium. a The human MSCs cultured with NBVbe medium had the different cell morphology from them cultured with the 5% PL medium. Scale bar, 100 μm. b Cell surface marker analysis by flow cytometry. c Colony formation assay for human MSCs cultured with the NBVbe medium or 5% PL medium. Scale bar for the upper panel, 1 cm. Scale bar for the lower panel, 200 μm. d Colony number counting for human MSCs cultured with the NBVbe medium or 5% PL medium for 5 passages. e The human MSCs were directed to adipocytes, osteocytes, and chondrocytes after cultured with the NBVbe medium for 5 passages. Left: Oil Red O staining and anti-FABP4 immuno-staining for the differentiated adipocytes; middle: Alizarin Red S staining and anti-Osteocalcin immunostaining for the differentiated osteocytes; right: Alcian blue staining and anti-Aggrecan immunostaining for the differentiated chondrocytes. Scale bar, 200 μm. An asterisk indicates P < 0.05