Fig. 3
1%O2 hMSCs suppress renal fibrosis more significantly than 21%O2 hMSCs in IRI rats. a, b Western blot analysis of α-SMA and TGF-β1 in the kidney cortex of IRI rats at day 21 post-IRI. Graph shows densitometric analysis of α-SMA and TGF-β1 expression levels normalized to the GAPDH expression level. c Representative immunohistochemical staining of α-SMA and collagen type I and III in kidney sections at day 21 post-IRI (scale bar = 100 μm). d Quantification of α-SMA- and collagen type I- and III-positive areas as percentages of the total area. e Representative images of HE, Masson trichrome, and Sirius red staining in kidney sections at day 21 post-IRI (scale bar = 100 μm). f Quantification of interstitial fibrosis area as percentages of the total area. Data are means ± S.D. #P < 0.01, *P < 0.05 (one-way ANOVA followed by Bonferroni’s post hoc test)