Fig. 6
VEGF siRNA transfection of 1%O2 hMSCs attenuates inhibition of TGF-β1-induced fibrotic changes in HK-2 cells. 1%O2 hMSCs were transfected with VEGF siRNA (VEGF siRNA/1%O2 hMSCs) or negative control siRNA (NC siRNA/1%O2 hMSCs). Concentrations of VEGF (a) and HGF (b) were measured in CM from VEGF siRNA/1%O2 hMSCs and NC siRNA/1%O2 hMSCs using ELISA kits. c ELISA analysis showing the concentration of HGF in CM from 21%O2 hMSCs stimulated by VEGF at various concentrations (time, 72 h). d Western blot analysis of α-SMA in HK-2 cells stimulated with TGF-β1 for 48 h. Graph shows densitometric analysis of the α-SMA expression level normalized to the α-tubulin expression level. Data are means ± S.D. #P < 0.01, *P < 0.05 (one-way ANOVA followed by Bonferroni’s post hoc test or Student’s t test)