Fig. 1

Cholesterol inhibits the proliferation and migration of TDSCs and induced G0/G1 phase arrest. a Cell viability was assessed by CCK-8 assay after treatment with various concentrations of cholesterol for different periods of time. b, c Cells were treated with 10 mg/dL cholesterol for 24 h. Ki67 expression was analyzed by immunofluorescence. Bar, 50 μm. d, e After treatment with 10 mg/dL cholesterol, the migratory capacity of TDSCs was assessed by wound healing assay. Bar, 100 μm. f, g Cells were treated with 10 mg/dL cholesterol for 24 h. The percentage of the cell population at G0/G1, S, and G2/M was analyzed by flow cytometry. All quantitative data are expressed as the means ± SEM of the results from three independent experiments. *p < 0.05 versus control. CHO, cholesterol