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Fig. 8 | Stem Cell Research & Therapy

Fig. 8

From: Epithelial differentiation of human adipose-derived stem cells (hASCs) undergoing three-dimensional (3D) cultivation with collagen sponge scaffold (CSS) via an indirect co-culture strategy

Fig. 8

Protein-expression analysis of K14 evaluated by immunofluorescence staining on day 15. Similar to HaCaT cells, hASCs-derived keratinocytes (differentiated hASCs) showed positive staining of K14 (green). Nucleus were stained with DAPI (blue). I Immunofluorescence staining images of glass slides seeded hASCs in each group. II Immunofluorescence staining images of CSSs containing hASCs (3D differentiation). III Typical image of glass slides seeded hASCs trypsinized from CSSs co-cultured at ALI, polygonal cells with keratinocyte-like morphology (white arrow), and multinucleated fused cells (white dotted circle) were observed. IV Quantification of K14 positive area (%) of CSSs containing hASCs, corresponding to II. V Quantification of K14 positive area (%) of glass slides seeded hASCs, corresponding to I ((A) hASCs indirectly co-cultured with HaCaT cells at ALI, (B) hASCs indirectly co-cultured with HaCaT cells under ALI, (C) hASCs cultured under ALI without HaCaT’s co-cultivation, (PC) HaCaT cells as positive control, (NC) undifferentiated hASCs as negative control) (*p < 0.05, **p < 0.01)

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