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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Loss of receptor tyrosine kinase-like orphan receptor 2 impairs the osteogenesis of mBMSCs by inhibiting signal transducer and activator of transcription 3

Fig. 4

Knockdown of Ror2 in mBMSCs by the use of recombinant lentivirus vectors. a mBMSCs transfected with empty lentiviral vector (mBMSC-mock1) and shRNA targeting Ror2 (mBMSC-sh-Ror2) were observed with light microscopy (top) and fluorescent microscopy (below). Scale bar = 100 μm. b Quantitative real-time PCR analysis showed Ror2 mRNA expression in mBMSCs after transduction. c, d The expression of Ror2 protein in mBMSCs after transduction were evaluated using western blot analysis. n = 3, *P < 0.05 vs. mBMSC-mock1

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Stem Cell Research & Therapy

ISSN: 1757-6512