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Table 2 Antibodies used for immunoblotting

From: Loss of receptor tyrosine kinase-like orphan receptor 2 impairs the osteogenesis of mBMSCs by inhibiting signal transducer and activator of transcription 3

Marker (species)

Dilution

Distributor/source (catalog number)

Primary antibody

 RANKL mouse mAb

1:100

Novus Biologicals (12A380)

 OSX rabbit mAb

1:70(IHF)

Santa Cruz (A13)

 OSX rabbit mAb

1:1000(WB)

Abcam (ab22552)

 OPN goat mAb

1:200(IHF)

R&D (af808)

 OPN rabbit pAb

1:1000(WB)

ZEN BIO(380437)

 COL1A1 goat pAb

1:200

Santa Cruz (sc-8784-R)

 CD31 rabbit mAb

1:200

Biolegend (102502)

 SOX9 rabbit mAb

1:300

Millipore (ab-5535)

 Ror2 rabbit pAb

1:1000

CST (4105)

 Stat3 mouse mAb

1:1000(WB)

CST (9139)

 Stat3 mouse mAb

1:100(ICF)

CST (9139)

 Runx2 rabbit mAb

1:1000

CST (8486)

 pStat3 mouse mAb

1:2000

CST (4113)

Secondary antibody

 Anti-rabbit IgG (Fluor® 488 Conjugate)

1:500 (IHF)

Yeasen Biotech (33106ES60)

 Anti-mouse IgG (Fluor® 568 Conjugate)

1:500 (IHF)

Abcam (ab175473)

 Anti-rat IgG (Fluor® 594 Conjugate)

1:500 (IHF)

Yeasen Biotech (34412ES60)

 Anti-mouse IgG (Fluor® 564 Conjugate)

1:300 (ICF)

Yeasen Biotech (33212ES60)

 Anti-mouse IgG HRP-linked Ab

1:2000 (WB)

CST (7076)

 Anti-rabbit IgG HRP-linked Ab

1:2000 (WB)

CST (7074)

  1. IHF immunohistofluorescence, WB western blot, ICF immunocytofluorescence