Fig. 6

Hierarchical clustering shows a close profile of gene expression for hISCs and BM-MSCs. DNA chips from hISCs, BM-MSCs, ASCs, and pancreatic islets were used to perform hierarchical clustering. Normalized microarray data (a total of 4390 genes) were plotted as a heat map, with columns corresponding to individual arrays and rows depicting the level of probe set signal expression from low (deep blue) to high (deep red) gene expression. Clustering is indicated by the dendrogram on top: all 5 hISC subpopulations strongly cluster with BM-MSCs and not with pancreatic islet (a). Hierarchical clustering shows genes encoding for MSC markers (b) and endocrine markers (c), in hISCs, BM-MSCs, and pancreatic islets. Contrary to pancreatic islets, hISCs as well as BM-MSCs weakly express endocrine markers and strongly express genes related to MSC profile. Top 10 gene set enrichment analysis (ordered by p value) of overexpressed genes in hISCs compared to pancreatic islets (d) and BM-MSCs (e) were generated with the Reactome Functional Interaction Cytoscape plugin. High-expression gene category in hISCs includes the organization and interaction of the ECM