Fig. 6
From: Paracrine effects of intraocularly implanted cells on degenerating retinas in mice
Identification of paracrine factors that nurture and/or protect retinal neurons. a Based on Affymetrix microarray gene expression data, nine out of 12 genes selected for qPCR were confirmed to be expressed in the cultured cells. b Total proteins were isolated from cell-conditioned media, separated by SDS-PAGE, transferred onto a PVDF membrane, and stained with 0.5% (w/v) Ponceau S. c Among the 5 selected genes [27,28,29,30,31,32], three encoded proteins were detected by WB to be present in the cell-conditioned media. d The neuroprotective effect of Vegfa was evaluated at the indicated time points by OKR after an intravitreal injection of the anti-VEGF antibody (bevacizumab) into RhoP23H/+ and e Rho+/+ mice at P40