Fig. 11

The impact of DPYSL2 knockout on the NSCs differentiation into neurons by immunofluorescent staining. The immunofluorescent staining of a, d, g nestin (red immunofluorescence), b, e, f Tuj1 (green immunofluorescence), and c, f, h GFAP (red immunofluorescence) in the +/+, DPYSL2^−/− Con group and DPYSL2^−/− PNS groups. DAPI was used to stain the cell nucleus (blue). Scale bar, 25 μm. i–k The representative bar graphs of the positive cells of nestin, Tuj1, and GFAP in these three groups. Data were presented as means ± SD. Each group contained 5 samples. *P < 0.05 versus DPYSL2^−/− Con group. Con, control group; PNS, Panax notoginseng saponins; +/+, wild type rats; −/−, DPYSL2-knockout rats