Fig. 3

The proliferation and differentiation of the cultured NSCs. a–d, b–e, c–f The immunofluorescent staining of nestin (red immunofluorescence), Tuj1 (green immunofluorescence), and GFAP (red immunofluorescence) in the serum and PNS + serum group. DAPI was used to stain the cell nucleus (blue). Scale bar, 25 μm. g–i The representative bar graphs of the proliferation and differentiation of NSCs or its differentiated states in the serum and PNS + serum groups. Data were presented as means ± SD. Each group contained 5 samples. *P < 0.05 versus Ser group. Ser, Serum group; P + S, PNS + serum group; Num, the number of the cells/neuronspheres; S, cell area; D, the length of the synapse. S/1000, S/10 represent the area value divided 1000 and 10, respectively