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Fig. 8 | Stem Cell Research & Therapy

Fig. 8

From: DPYSL2 is a novel regulator for neural stem cell differentiation in rats: revealed by Panax notoginseng saponin administration

Fig. 8

Construction and identification of the ORF-DPYSL2 lentivirus. a Information of the EX-Rn10192-Lv201recombinant. b Enzyme digestion products of EX-Rn10192-Lv201recombinant, separated by 3% agarosegel electrophoresis. Lane 1, 4, and 5: DNA Ladder. Lane 2: EX-Rn10192-Lv201 plasmid. Lane 3: EX-Rn10192-Lv201 plasmid digested by NspVandXhoI. c Represent NSCs pictures taken by inversed fluorescent microscope before and at 3 and 7 days post transfection. Green: eGFP positive cells. Scale bar = 50 μm. Nor, normal group, neural basal medium addition; eGFP, eGFP group, eGFP-lentivirus transfection; DPYSL2, DPYSL2 group, DPYSL2-ORF vector transfection

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Stem Cell Research & Therapy

ISSN: 1757-6512