Fig. 9

The detection of NSCs differentiation toward neurons and astrocytes after DPYSL2-ORF transfection. a–c, e–g, i–k The immunofluorescent staining of DPYSL2 (red immunofluorescence), Tuj1 (green immunofluorescence), and GFAP (red immunofluorescence), respectively, in the three groups (Nor, eGFP, DPYSL2) at 7 days after transfection, DAPI stained the cell nucleus (blue). d, h, l The representative bar graphs for the ratio of the positive cells in the three groups. Data were presented as means ± SD. *P < 0.05. Scale bar, 25 μm. Each group contained 5 samples