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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: PTEN inhibitor VO-OHpic attenuates GC-associated endothelial progenitor cell dysfunction and osteonecrosis of the femoral head via activating Nrf2 signaling and inhibiting mitochondrial apoptosis pathway

Fig. 1

VO-OHpic significantly attenuates the cytotoxicity of MPS on EPCs. a The flow cytometry analysis shows that EPCs positively express CD34, VEGFR2, and CD133. b The cytotoxicity of MPS on EPCs viability was evaluated with CCK-8 at the concentrations of 0, 10, 20, and 50 μM after 48 h. c VO-OHpic effectively attenuates the detrimental effects of MPS (50 μM) on EPCs viability at concentrations of 0.1, 1, and 10 μM. d P-AKT and t-AKT protein levels were determined by western blot analysis at 48 h. e Band density ratios of p-AKT to t-AKT in the western blots were quantified by densitometry. All experiments were repeated for three times; *P < 0.05 versus control; #P < 0.05 versus 50 μM MPS group; Error bar represent SD

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Stem Cell Research & Therapy

ISSN: 1757-6512