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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: PTEN inhibitor VO-OHpic attenuates GC-associated endothelial progenitor cell dysfunction and osteonecrosis of the femoral head via activating Nrf2 signaling and inhibiting mitochondrial apoptosis pathway

Fig. 5

VO-OHpic significantly abrogates the inhibitory effect of MPS on the Nrf2 signaling pathway in EPCs. a Sirt1, Nrf2, HO-1, NQO-1, and Trx protein levels in EPCs were determined by western blot analysis after treatment with 50-μM MPS or 50-μM MPS combined with 1-μM VO-OHpic for 48 h. b Band density ratios of Sirt1, Nrf2, HO-1, NQO-1, and Trx to GAPDH in the western blots were quantified by densitometry. c Immunofluorescence staining of EPCs cultured with 50-μM MPS or 50-μM MPS combined with 1-μM VO-OHpic for 48 h. Nrf2 subcellular localization was determined by immunofluorescence staining for Nrf2 (red) along with DAPI for DNA (blue) at × 400 magnifications. d Cytoplasmic Nrf2 protein expression was determined by western blot analysis, and GAPDH was selected as the internal control. Nuclear Nrf2 protein expression was determined by western blot analysis, and Lamin B was selected as the internal control. Band density ratios were also quantified by densitometry. All experiments were repeated for three times; ∗P < 0.05 versus control, #P < 0.05 versus 50-μM MPS group. Error bar represents SD

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Stem Cell Research & Therapy

ISSN: 1757-6512