Fig. 2

MSCs co-cultured with SCs. Scale bar, 20 μm. Morphological analysis and distribution of MSCs co-cultured with SCs or acellular control evaluated after 4 days as shown by immunofluorescence imaging with anti-S00 (green, a, d, g, j) and DAPI (blue, b, e, h, k) and their merged micrographs (c, f, i, l). a–c ADSCs co-cultured with SCs. d–f BMSCs co-cultured with SCs. g–i ADSC-only culture (control). j–l BMSC-only culture (control). Histogram (m) showing comparisons of the number of S100-positive cells as a percentage of DAPI-positive nuclei in the SCs-MSC co-culture system. *p < 0.05 versus single-cell/mono-cultured group, ^§p > 0.05 versus BMSC group