Fig. 3

Characterisation of proliferating CDC, DNC and CSPC populations. a Colony forming efficiency (CFE; left axis—red) of CSPCs from nine donors and number of cells/colony (right axis—black) after 14 days in culture. Upper panel insert: representative crystal violet staining of CSPC colonies. Scale bars = 10 mm and 100 μm (left to right). b Population doublings (PD) of CDC, DNC and CSPC cultures along cell passages (P1–P13). Inset represents patient variability (n = 5 at passage 4). c Changes in cell index over time according to RTCA iCELLigence™ assay (n = 3) with bar graphs to demonstrate maximum cell index reached after 24 h in culture, using RTCA iCELLigence™ assay (n = 3). Results as mean ± SEM, ***p < 0.001. d G-band karyotype analysis of CDCs, DNCs and CSPCs at P4 (46XY) representative analysis from 5 human donors analysed for changes at P1 vs P4