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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Characterisation of extraembryonic endoderm-like cells from mouse embryonic fibroblasts induced using chemicals alone

Fig. 1

Generation of ciXEN cells. a Schematic diagram for obtaining ciXEN cells from MEFs using small molecules. b Morphological appearances of MEFs, cell colonies (treatment with chemicals for 8 days (8d) and 16 days (16d)) (bar, 100 μm), and epithelial-like cells emerging out of the selected clone (24d) (bar, 200 μm). c Numbers of cell clones with different numbers of starting cells: 3w, 4w, and 5w. d Effect of Matrigel on the formation of clones from MEFs with 4w initial cells. e Co-immunostaining (Foxa2 and Sox17) and phase-contrast image of cell clone (bar, 50 μm). qPCR results for the expression of XEN-related genes and Sox2 (f), and fibroblast markers and epithelial markers (g) from day 0 to day 24. V, VPA; P, Parnate; A, AM580; C, CHIR99021; R, RepSOX; F, forskolin; E, EPZ004777; 3w, 3 × 104 cells; 4w, 4 × 104 cells; 5w, 5 × 104 cells. *p < 0.05, **p < 0.01, ***p < 0.001. Mean ± SEM, t test and two-way ANOVA, n ≥ 3

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