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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Characterisation of extraembryonic endoderm-like cells from mouse embryonic fibroblasts induced using chemicals alone

Fig. 6

Generation of iHeps from ciXEN cells induced by staged cytokines. a Cell morphology of iHeps. The black arrow points to binuclear or multinuclear cells (bar, 100 μm (left) and 50 μm (right)). b Immunofluorescence of hepatic genes Alb, Afp, and Hnf4a in iHeps (bar, 50 μm). c, d Expression of hepatic genes detected by RT-PCR/qPCR. e mRNA levels of XEN genes (Sox17, Gata4, Gata6, and Foxa2) and Sox2 in iHeps as determined by qPCR. mH, mouse hepatic tissue. f Functions of iHeps (glycogen storage and ICG uptake and release) detected by PAS staining and ICG assay, respectively (bar, 100 μm). *p < 0.05, **p < 0.01, ***p < 0.001. Mean ± SEM, t test, n = 3

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