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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: Characterisation of extraembryonic endoderm-like cells from mouse embryonic fibroblasts induced using chemicals alone

Fig. 7

The characteristics of iXEN cells derived from ciXEN cells by withdrawing chemicals. The cell morphologies of iXEN cells (bar, 100 μm) (a). b, c Immunostaining of Gata4, Sox2, Foxa2, Sox17, vimentin, and E-cadherin in iXEN cells (bar, 50 μm). d Western blot analysis for the expression of Foxa2, Sox17, Gata4, Sox2, E-cadherin, and vimentin in MEFs, ciXEN cells, and iXEN cells. qPCR results for the expression of XEN genes (Sox17, Gata4, Gata6, and Foxa2) (e) and Sox2 (f), fibroblast markers (Prrx1, Col1a1, Pdgfrb, and Thy1) (g), and epithelial genes and mesenchymal-related genes (h). *p < 0.05, **p < 0.01, ***p < 0.001. Mean ± SEM, two-way ANOVA, n = 3

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Stem Cell Research & Therapy

ISSN: 1757-6512