Fig. 6From: Phenotype instability of hepatocyte-like cells produced by direct reprogramming of mesenchymal stromal cellsEvaluation of liver repopulation capacity of iHEPs in the APAP acute liver injury model. Experimental design (a). Confocal microscopy of liver sections obtained from iHEP-transplanted mice 48 h (b) or 7 days (c) after cell transplantation. iHEPs are visualized by GFP expression (green), while albumin or CD31+ vessels are seen in red. Nuclei were stained with DAPI (blue). Inset shows a detail for observation of Alb/GFP double staining. Scale bars 100 μm (b, c; right), 200 μm (c; left), 50 μm (c; middle). Representative images of H&E-stained liver sections showing extensive areas of centrolobular necrosis 48 h after APAP injection (d; left) and complete restoration of normal architecture 7 days after APAP injection (d; right), with no signs of ectopic proliferationBack to article page