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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Vitamin C improves the therapeutic potential of human amniotic epithelial cells in premature ovarian insufficiency disease

Fig. 2

VC promoted the migration ability and extended the life span of hAECs. a Wound healing assay results showing the migratory ability of hAECs after treatment with 25, 50, and 100 μg/ml VC. The yellow dotted lines depict the boundaries of the wound. b hAEC viabilities measured at passage 1 (P1), P5, and P10 after treatment with 25, 50, and 100 μg/ml VC from P1. Cell viability assay results at different passages are summarized on the right. Cell viability was determined by trypan blue. Scale bar = 20 μm. c Cell cycle analysis of hAECs at passage 5 showing G1 phase, S phase, and G2 phase fractions after treatment with 25, 50, and 100 μg/ml VC. The percent hAECs in the G0/G1, S, and G2/M phases are summarized below. d WB results indicating the protein levels of hTERT in hAECs at passage 5 after treatment with 25, 50, and 100 μg/ml VC. PBS was added to the culture media of the hAECs as a control

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