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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Vitamin C improves the therapeutic potential of human amniotic epithelial cells in premature ovarian insufficiency disease

Fig. 3

VC treatment promoted pluripotency marker expression of hAECs. a, b FACS results illustrating the percent OCT4+, NANOG+, SSEA4+, and TRA-1-81+ hAECs and VC-hAECs at passage 1 and passage 5. c Alkaline phosphatase (AP) staining images of hAECs and VC-hAECs at passage 5. The relative fluorescence intensity was measured and is presented on the right. Paired t test, ***p < 0.001, versus hAEC group. d, e Protein-level assay results showing pluripotency marker (OCT4, NANOG, SSEA4, and TRA-1-81) expression levels in hAECs and VC-hAECs at passage 1 and passage 5. VC-hAECs = hAECs treated with VC

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Stem Cell Research & Therapy

ISSN: 1757-6512