Fig. 4

Runx1t1 gene expression level in the Runx1t1 overexpression group was higher than that in the mock-infected and NC groups (a). The level of Runx1t1 protein in the Runx1t1 overexpression group was also significantly greater than that in the mock-infected and LV4-NC groups (b). The difference between the Runx1t1 overexpression group and the other groups was statistically significant. Thus, the NSCs from the hippocampus infected with LV4-Runx1t1 showed effectively upregulated Runx1t1 protein and gene expression. Compared to the mock-infected and LV4-NC groups, a large number of Tuj1-positive cells were detected in LV4-Runx1t1, and the length of processes of Tuj1-positive cells increased (a–c). Scale bar = 50 μm. The numbers of Tuj1-positive cells showed a significant difference between mock-infected and LV4-NC groups with an LV4-Runx1t1 group (l). P < 0.05