Fig. 6

The dotted box showed the injection tract (a, b). Several EGFP-positive cells gathered near the track. The upper cells in the granular and subgranular zones of DG were transfected notably. Thus, the square region (shown in a–d) was the area of interest in immunofluorescence staining analyses for the subsequent studies. Real-time PCR revealed that Runx1t1 gene expression level in the LV4-Runx1t1 group was significantly higher than that in the LV4-NC group (k). The protein level of Runx1t1 in the LV4-Runx1t1 group was also higher than that in the LV4-NC group, which presented weak expression (l). The number of Runx1t1 immunopositive cells in the LV4-Runx1t1 group was also significantly than that in the LV4-NC groups (e–j, m). After 14 days, immunocytochemistry was performed. Extensive DCX immunoreactivity was detected in the LV4-Runx1t1 group (n–s). The number of DCX-positive cells was significantly more, and the cell processes were longer than that in the LV4-NC group (t). Scale bar = 50 μm, P < 0.05