Fig. 1
Characterization, internalization of EVs, and analysis of EV release from collagen matrix. a TEM image of EVs. Scale bar, 100 nm. b Western blot analysis of the marker of EVs, CD9, Alix, and TSG101 in EVs, MSCs, and EV-free conditioned medium. c Size distribution measure of EVs by NTA in scatter mode. d, e Internalization of Gluc-labeled EVs by HK-2 cells at various time points was analyzed with BLI. f, g The released EVs were determined by quantitative analysis of the Gluc signal. h Retention ratio of EVs in Col-EVs after immersing in PBS within 12 h (R2 = 0.8525). The signal activity was represented by photons/s/cm2/steradian