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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Macrophage M2 polarization induced by exosomes from adipose-derived stem cells contributes to the exosomal proangiogenic effect on mouse ischemic hindlimb

Fig. 2

M1 macrophages are polarized to an M2-like phenotype by exosomes from ASCs. a, b Macrophage intracellular markers, iNOS for M1 and Arg-1 for M2, in M1 that were treated with the indicated concentration of Nor/Exo or Hyp/Exo for 48 h (a) or treated with 30 μg/ml Nor/Exo or Hyp/Exo for the indicated times (b) were examined by using immunoblotting. GAPDH was used as a loading control (n = 3). c–f Macrophage surface markers, CD86 (c) for M1 and CD206 (e) for M2, on M1 that were treated with 30 μg/ml Nor/Exo or Hyp/Exo for 48 h were examined by using flow cytometry (n = 3). Panels d and f are the statistical results of panels e and g, respectively. F4/80 was used as a pan-macrophage marker. M0 was used as a negative control. M2 was used as a positive control. **p < 0.01 and ***p < 0.001. NS, not significant

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