Fig. 4

Exosomes from ASCs promote angiogenesis in a mouse Matrigel plug assay. Matrigel containing Nor/Exo or Hyp/Exo was subcutaneously injected into the flanks of mice (n = 6). The Matrigel plugs were harvested 2 weeks post implantation. a Representative images of the plugs are shown. b The sections of the plugs were subjected to immunohistochemistry analysis for CD31, an endothelial cell marker. Scale bar = 100 μm. Quantification of the CD31⁺ area was performed. The positive area in the slide with plugs containing PBS was set to 1. c, d Cells collected from the plugs were subjected to flow cytometry analysis. The percentage of F4/80⁺ cells in the total analyzed cells is displayed (c). The percentage of CD206⁺, an M2 macrophage marker, cells in F4/80⁺ cells was further analyzed (d). *p < 0.05 and **p < 0.01