Fig. 4

The neotissue characteristics in the ACWJ group were similar to the surrounding hyaline cartilage. a, b The results of H&E staining and Safranin “O” staining of the repair area of femoral condyles, respectively. The images below are the dotted box partial enlargement of the image above, the scales of black and white bar equal to 2 mm and 0.8 mm, respectively. c, d collagen I and collagen II immunohistochemistry staining of repair area of femoral condyles. e The results of the International Cartilage Repair Society Visual-Histological Assessment Scale for Cartilage Repair: ICRS-VHAS, ICRS- visual histological assessment scale; ICRS-SB, ICRS-subchondral bone; ICRS-CD, ICRS-cell distribution; ICRS-M, ICRS-matrix; n = 3. Data are expressed as the mean ± standard deviation; black circles, black squares, black triangles and black stars indicate the significant differences between the ACCC group and the BC, AC, ACAC and ACWJ groups. P < 0.05 indicates a statistically significant difference