Fig. 1

LSD1 expression changes during human embryonic stem cell differentiation into insulin-producing β cells. a Schematic of a step-wise differentiation protocol of hESCs into insulin-producing β cells. ES, embryonic stem cell; DE, definitive endoderm cells; PP1, PDX1⁺NKX6.1⁻ pancreatic progenitor cells; PP2, PDX1⁺NKX6.1⁺ pancreatic progenitor cells; IPC, insulin-producing cells. b Representative immunofluorescence images stained with marker genes of different stages. c Relative mRNA expression of LSD1 at different differentiation stages. d Representative Western blot images of LSD1 protein level at the different differentiation stages