Fig. 2
From: LSD1 inhibition yields functional insulin-producing cells from human embryonic stem cells
Effect of LSD1 knockdown or inhibition on hESCs differentiation into DE cells. a Diagram of experimental design for c–f, i, and k. b Diagram of experimental design for g, h, and j. c Protein levels of LSD1, oct4, and Nanog detected by Western blot at the end of DE d Relative mRNA expression of pluripotency genes COT4, SOX2, and Nanog in ES treated with LSD1 shRNAs. e The co-expression of SOX17 with FOXA2 was detected by immunofluorescence assay at the definitive endoderm stage in control and LSD1-shRNA group (e), control and TCP group (g), respectively. f, h–j Effects of LSD1 shRNAs (f) and TCP (h) during definitive endoderm differentiation on SOX17 and CXCR4 expression and quantification of the proportion of SOX17+/CXCR4+ (i, j) by flow cytometry. k The mRNA expression of DE-associated genes SOX17, FOXA2, and CXCR4 in control and LSD1 shRNA groups. Data represent mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001, two-sided student’s t test (n = 3 biologically independent samples per group)