Fig. 3
From: LSD1 inhibition yields functional insulin-producing cells from human embryonic stem cells
LSD1 knockdown or inhibition enhances the generation of PP2 cells. a Diagram of experimental design for d, e, and h. b Diagram of experimental design for f, g, and i. c Relative mRNA expression of pancreatic progenitor associated genes detected by real-time PCR at the end of PP2 after LSD1 knockdown (d, f). The co-expression of PDX1 with NKX6.1 was detected by immunofluorescence assay at stage 3 in control and LSD1-shRNA group (d) and control and TCP group (f), respectively. Effects of LSD1 shRNAs (e, h) and TCP (g, i) on the co-expression of PDX1 and NKX6.1 during pancreatic progenitor differentiation and quantification of the proportion of PDX1+/NKX6.1+, respectively. Data represent mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001, two-sided student’s t test (n = 3 biologically independent samples per group)