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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: LSD1 inhibition yields functional insulin-producing cells from human embryonic stem cells

Fig. 4

ERK signaling was activated during PP2 specification. a The protein levels of LSD1 and phosphorylated and total ERKs were analyzed by Western blot after LSD1 shRNA treatment. Actin was used as an internal control to verify equal protein loading. b Effects of the expression of control shRNA and LSD1 shRNA during pancreatic progenitor differentiation on NKX6.1 and CHGA expression and quantification of the proportion of NKX6.1−/CHGA + (c) cells from b as assayed at the end of stage 3. d The protein levels of phosphorylated and total ERKs were analyzed by Western blot after TCP treatment. Flow cytometry analysis of NGN3 expression at stage 3 (e) and quantification of the proportion of NGN3+ cells (f) from e. Flow cytometry analysis of NKX6.1 and the proliferation marker Ki67 in control and TCP-treated pancreatic progenitors (g) and quantification of co-expression of PDX1 and Ki67 in PP2 cells (h) from g as assayed at the end of stage 3. Flow cytometry analysis of PDX1 and the proliferation marker Ki67 in control and TCP-treated pancreatic progenitors (i), and quantification of co-expression of PDX1 and Ki67 in PP2 cells (j) from i as assayed at the end of stage 3. Data represent mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001, two-sided student’s t test (n = 3 biologically independent samples per group)

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