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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Inflammatory priming enhances mesenchymal stromal cell secretome potential as a clinical product for regenerative medicine approaches through secreted factors and EV-miRNAs: the example of joint disease

Fig. 4

ASC-EV characterization. a Number of EVs secreted per cell in 48 h. b Transmission electron micrographs of ASC-derived vesicles showing particles with characteristic cup-shaped morphology. c Size distribution of nanoparticles by NanoSight particle tracking analysis. d Mean particle size analysis from NTA data. e Setting up the EV-dedicated flow cytometer. The resolution of the reference bead mix indicates the flow cytometer performance in light scattering at default settings. The top cytogram shows the side scatter height (SSC-H) versus forward scatter area (FSC-A). The bottom cytogram depicts the SSC-H versus 535/35 (green fluorescence triggering) channel. Four fluorescent populations (100, 300, 500, and 900 nm) were resolved from the instrument noise. f Flow cytometry analysis of ASC-EVs and iASC-EVs. EVs were stained with CFSE to allow identification and gating of vesicles in the FITC channel. After gating, CFSE+ EVs showed positive extracellular vesicle defining molecules CD63 and CD81. Representative cytograms are presented

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Stem Cell Research & Therapy

ISSN: 1757-6512