Fig. 8

AD-MSC-conditioned medium administration improves the angiogenesis of the wounds in diabetic mice. a, b Representative confocal images of histological sections of the wound obtained from 26-week-old non-diabetic and diabetic mice treated with vehicle, conditioned medium derived from non-preconditioned AD-MSCs, or conditioned medium derived from DFX-preconditioned AD-MSCs, 14 days after wound induction. Blood vessels were stained with BS-1Lectin (a) or VE-cadherin (b) and nuclei were stained with DAPI. c, d Quantification of the vascular area in the wound 14 days after wound induction for BS1-Lectin labeling (c) or VE-cadherin labeling (d). e Quantification of mRNA levels of pro-angiogenic factors IGF-1, angiopotetin-1, PDGF, and VEGF-α in the wound bed, 7 days after wound induction. Data were normalized against the housekeeping gene β-actin. f Representative confocal images of histological sections of the wound, 14 days after wound induction. Proliferating cells in the wound were detected with anti-Ki67 antibody. Nuclei were stained with DAPI. Arrows indicates Ki67 positive cells. g Quantification of proliferating cells in the wound 14 days after wound induction. Data are presented as percentage of Ki67 cells per square millimeter of wound area. Quantitative data are presented as mean ± S.E.M. (n = 6, one-way ANOVA with Tukey post-test, *p < 0.05)