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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Regenerative and protective effects of dMSC-sEVs on high-glucose-induced senescent fibroblasts by suppressing RAGE pathway and activating Smad pathway

Fig. 2

HG accelerated fibroblast senescence. a HG-induced ROS generation in DFs. HDFs were treated with HG for 1, 3, 5, and 7 days, and then the cells were labeled with DCFH-DA, followed by fluorescence microscopy. Scale bar = 100 μm. b ROS generation in HDFs labeled with DCFH-DA and analyzed by flow cytometer. The fluorescence intensity is expressed as arbitrary units (a.u.) (n = 5). c, d SA-β-gal-kit was adopted to evaluate the SA-β-gal assay in HDFs (n = 5). Scale bar = 100 μm. e The expression level of p21 and the quantification result. The results were normalized to GAPDH expression (n = 5). ***p < 0.001, ****p < 0.0001

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