Fig. 5

Comparison of gene expression changes upon 2D and 3D culturing of equine tenocytes. Comparison of RNA transcriptome profile between three biological lines of fetal and adult tenocytes cultured in either a 2D or 3D. a Euclidean sample-to-sample distance matrix with hierarchical clustering using VST transformed data. b Principle component analysis of gene expression data profiling three biological lines of 3D versus 2D cultured adult and fetal tenocytes. c Quantitative VENN diagram showing overlap of DE genes in the adult 3D vs 2D culture comparison (teal) and fetal 3D vs 2D culture comparison (orange). Top 4 upregulated genes based on their p-adj value are shown in both groups next to the green arrow and the top 4 downregulated genes value are shown in both groups next to the red arrows