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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Inhibition of LSD1 promotes the differentiation of human induced pluripotent stem cells into insulin-producing cells

Fig. 4

IP-WB and ChIP-qPCR assessment of the effects of LSD1 knockdown on promoter complexes. a LSD1 and HDACI coexistence with the CoREST complexes was confirmed with IP-WB (NRS as normal rabbit serum, control group). b After shRNA-LSD1-927 knockdown, LSD1/CoREST/HDAC1 complexes in the SOX17/FOXA2 gene promoter regions show differences. c After shRNA-LSD1-927 knockdown, the histone modification levels of H3K4me2/me3 in the OCT4/SOX2 gene promoter regions show differences. d After shRNA-LSD1-927 knockdown, the histone modification levels of H3K4me2/me3 in the SOX17/FOXA2 gene promoter regions show differences. e After shRNA-LSD1-927 knockdown, the histone modification levels of H3K9act in the SOX17/FOXA2 gene promoter regions show differences (*P < 0.05; **P < 0.01, n = 3)

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