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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Bone marrow-derived from the human femoral shaft as a new source of mesenchymal stem/stromal cells: an alternative cell material for banking and clinical transplantation

Fig. 4

Analysis of BM-MSC cell growth. a Population doubling time (PDT, measured in days) determined at each culture of BM-MSCi, BM-MSCt, and BM-MSCc from the 1st to passage 7. b The comparison of the growth curves of different BM-MSCs (passage 2/3) calculated based on metabolic activity determined by measuring absorbance at 450 nm wavelength. c The representative images of senescence β-galactosidase-positive cells in BM-MSCi, BM-MSCt, and BM-MSCc taken from pictures of five independent experiments. Light blue staining can be observed from passage 7 and gradually increases at passage 11. d. Graph representing the percentage of proliferating cells stained with Ki67 antibody. All data reflect mean ± SD from ≥ 5 independent experiments; **p < 0.01

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