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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: LncRNA H19 promotes odontoblastic differentiation of human dental pulp stem cells by regulating miR-140-5p and BMP-2/FGF9

Fig. 2

H19 promotes odontogenesis of hDPSCs. a Fluorescence was observed under an inverted fluorescence microscope after transfection for 48 h. b The expression levels of H19 were determined by qRT-PCR. c Relative mRNA expressions of DMP-1 and DSPP measured by qRT-PCR after odontoblast induction for 7 days. d Western blot results showed the protein levels of DSPP and DMP-1 decreased in shH19-1 and shH19-2 groups after odontoblast induction for 7 days. e The ALP activity was decreased by H19 knockdown after odontoblast induction for 7 days. f Images of Alizarin Red S staining in sh-NC, shH19-1, and shH19-2 groups. hDPSCs were cultured in odontoblastic medium for 7 days. g H19 was upregulated after hDPSCs were transfected with pcDNA 3.1-H19. h qRT-PCR assay revealed that the mRNA expressions of DMP-1 and DSPP were increased in H19 overexpression group after odontoblast induction for 7 days. i Western blot analysis of protein expression of DMP-1 and DSPP in hDPSCs after odontoblast induction for 7 days. j After odontoblast induction for 7 days, Alizarin Red S staining showed that H19 overexpression group generated more calcified nodules than control group. k The ALP activity was increased by H19 overexpression after odontoblast induction for 7 days. The data are presented as the mean ± SD in three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001

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