Fig. 5

H19 regulates BMP-2/FGF9 expression by binding to miR-140-5p. a Schematic of BMP-2/FGF9 wild-type (WT) and mutant (Mut) luciferase reporter vectors. b miR-140-5p significantly reduced luciferase activity in BMP-2/FGF9-wild not in BMP-2/FGF9-mut. c Quantification of mRNA expression of BMP-2 and FGF9 measured by qRT-PCR in miR-140-5p mimics, pcDNA3.1-H19, miR-140-5p mimics/pcDNA3.1-H19 co-transfected hDPSCs relative to the control groups. d Relative expression of BMP-2 and FGF9 in hDPSCs measured by ELISA in miR-140-5p mimics, pcDNA3.1-H19, and miR-140-5p mimics/pcDNA3.1-H19 co-transfected hDPSCs. e Relative expression of BMP-2 and FGF9 in hDPSCs measured by ELISA after odontoblast induction for 14 days. f Relative mRNA expressions of ALP, RUNX2, DSPP, and DMP-1 in exogenous BMP-2-treated groups and exogenous FGF9-treated groups after odontoblast induction for 14 days. g Relative protein expressions of RUNX2, DSPP, and DMP-1 in exogenous BMP-2-treated groups and exogenous FGF9-treated groups after odontoblast induction for 14 days. h Representative micrographs of Alizarin Red S staining in exogenous BMP-2-treated groups and exogenous FGF9-treated groups after odontoblast induction for 14 days. The data are presented as the mean ± SD in three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001