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Table 2 Antibody list

From: Generation of gene-corrected functional osteoclasts from osteopetrotic induced pluripotent stem cells

Antibodies

Catalog number

Company

Dilution

Immunostaining

 Mouse anti-oct3/4

sc5279

Santa Cruz

1:50

 Rabbit anti-Nanog

ab21624

Abcam

1:150

 Mouse anti-TRA-1-60

MAB4360

Merck

1:100

 Rabbit anti-SOX2

AB5603

Merck

1:500

 Alexa Fluor 488 Donkey anti-mouse IgG (H+L)

A21202

Thermo Fisher Scientific

1:2000

 Alexa Fluor 568 Donkey anti-rabbit IgG (H+L)

A10042

Thermo Fisher Scientific

1:2000

 Alexa Fluor 647 Donkey anti-rabbit IgG (H+L)

A31573

Thermo Fisher Scientific

1:2000

Western blot

 Mouse anti-human TCIRG1

H00010312

Abnova

1:1000

 Rabbit anti-p38 MAPK

9212

Cell Signaling Technology

1:1000

 ECL Mouse IgG, HRP-linked whole Ab (from sheep)

NA9310

GE Healthcare

1:5000

 ECL Rabbit IgG, HRP-linked F(ab′)2 fragment (from donkeys)

NA9340

GE Healthcare

1:5000