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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Extracellular vesicles derived from human ES-MSCs protect retinal ganglion cells and preserve retinal function in a rodent model of optic nerve injury

Fig. 1

Isolation and characterization of embryonic stem cell-derived mesenchymal stem cells (ES-MSC) extracellular vesicles (EVs). a Phase contrast micrograph of passage-9 human embryonic stem cell-derived mesenchymal stem cells (ES-MSCs). b Extracellular vesicles (EVs) were isolated from conditioned medium of human ES-MSCs using differential ultracentrifugation. The supernatants (Sup.1–3) were submitted to further centrifugation and the pellets (Pel. 1–3) and Sup. 4 were discarded. c Scanning electron microscopy (SEM) of the EVs. d Size distribution of ES-MSC EVs was assessed using dynamic light scattering (DLS). Size quantification data demonstrated an average size of 130 nm for the EVs. e The Coomassie stained gel of loaded EVs. f Representative Western blot analysis for expression of enriched proteins in EVs (two EV membrane markers [CD63 and CD81], and one laminal marker [TSG101])

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