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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Mitochondria transfer enhances proliferation, migration, and osteogenic differentiation of bone marrow mesenchymal stem cell and promotes bone defect healing

Fig. 1

Uptake of donor mitochondria by recipient BMSCs increased in a dose-dependent manner. Confocal immunofluorescence staining of nuclei with DAPI in BMSCs from the control group (a) and treatment group (b), and F-actin with FITC-labeled phalloidin in BMSCs from the control group (c) and treatment group (d) at 24 h after mitochondria transfer. Confocal immunofluorescence staining of transferred mitochondria labeled before isolation from the control group (e) and treatment group (f) at 24 h after mitochondria transfer. g, h Merged confocal images of a, c, and e or b, d, and f, respectively. Scale bar represents 50 μm. Confocal immunofluorescence imaging of treatment groups containing amplified xZ-stack and yZ-stack images (i). j FACS analysis of the number of labeled mitochondria (displayed as fluorescence intensity per cell) within recipient cells of each group. k Linear regression showing linear relationship between relative MFI values and labeled mitochondria number. Results are presented as mean ± SEM (n = 3)

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