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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Mass spectrometric analysis of the in vitro secretome from equine bone marrow-derived mesenchymal stromal cells to assess the effect of chondrogenic differentiation on response to interleukin-1β treatment

Fig. 1

Schematic drawing of the workflow. a Bone marrow-derived mesenchymal stromal cells (BM-MSCs) were isolated from the equine sternal bone marrow. b BM-MSCs were expanded to P4. c The BM-MSCs were seeded in 6-well plates and divided in the following treatment groups: (1) expansion media without IL-1β (EM), (2) expansion media with IL-1β (EMIL) for 5 days followed by 5 days recovery time, (3) chondrogenic media without IL-1β (CM), and (4) chondrogenic media with IL-1β (CMIL) for 5 days followed by 5-day recovery time. Media in the 6 wells were harvested and pooled from each of the treatment groups after 48 h and 10 days. d Samples were concentrated in spin filters, processed, analyzed by mass spectrometry (MS), and the acquired data interpreted

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