Fig. 1

Elevation of miR-22-3p enhances osteogenic differentiation of BMSCs. a BMSCs were cultured in NM and OM for 14 days for ALP staining (× 400). b BMSCs were cultured in NM and OM for 14 days for ARS staining (× 200). c BMSCs were cultured in NM and OM for 14 days, and RT-qPCR was used to detect miR-22-3p expression. d Transfection efficiency examination was determined using RT-qPCR in the mimic-NC miR-22-3p mimic, inhibitor-NC, or miR-22-3p inhibitor groups. e BMSCs were stained by ALP (× 400). f BMSCs was stained by ARS staining in the mimic-NC miR-22-3p mimic, inhibitor-NC, or miR-22-3p inhibitor groups (× 200). g RT-qPCR analysis of RUNX2, OCN, and OPN mRNA expression in BMSCs in the mimic-NC miR-22-3p mimic, inhibitor-NC, or miR-22-3p inhibitor groups. h Immunoblotting was adopted to determine RUNX2, OCN, and OPN protein expression in BMSCs in the mimic-NC miR-22-3p mimic, inhibitor-NC, or miR-22-3p inhibitor groups. **p < 0.001 vs. the NM-14 days group; *p < 0.05 vs. the mimic NC group; ^#p < 0.05 vs. the inhibitor-NC group. Comparison between two groups was performed by unpaired t test