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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Extracellular vesicle-encapsulated miR-22-3p from bone marrow mesenchymal stem cell promotes osteogenic differentiation via FTO inhibition

Fig. 3

Downregulation of EV-encapsulated miR-22-3p impairs BMSC osteogenic differentiation. a The ALP activity of BMSCs were determined using ALP staining in the PBS, BMSC-EV, BMSC-EV/inhibitor-NC, or BMSC-EV/miR-22-3p inhibitor groups (× 400). b The extracellular matrix mineralization was determined using ARS staining in the PBS, BMSC-EV, BMSC-EV/inhibitor-NC, or BMSC-EV/miR-22-3p inhibitor groups (× 200). c, d RT-qPCR and Immunoblotting were adopted to determine the mRNA and protein level of RUNX2, OCN, and OPN in the PBS, BMSC-EV, BMSC-EV/inhibitor-NC, or BMSC-EV/miR-22-3p inhibitor groups. e miR-22-3p expression in BMSCs of the sham, OVX + PBS, OVX + BMSC-EV, OVX + BMSC-EV/inhibitor-NC, or OVX + BMSC-EV/miR-22-3p inhibitor groups. f ALP activity of BMSCs in mice was determined using ALP staining in the sham, OVX + PBS, OVX + BMSC-EV, OVX + BMSC-EV/inhibitor-NC, or OVX + BMSC-EV/miR-22-3p inhibitor groups (n = 12/group) (× 400). g ARS staining was adopted to determine the extracellular matrix mineralization of BMSC in mice of the sham, OVX + PBS, OVX + BMSC-EV, OVX + BMSC-EV/inhibitor-NC, or OVX + BMSC-EV/miR-22-3p inhibitor groups (× 200) (*p < 0.05 vs. the sham group or the BMSC-EV/inhibitor-NC group, #p < 0.05 vs. the PBS group or the OVX + PBS group, and p < 0.05 vs. the OVX + BMSC-EV/inhibitor-NC group). Comparison of data between multiple groups was performed by one-way ANOVA. Tukey’s was used for post hoc tests

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