Fig. 4

miR-22-3p inversely targets FTO. a Venn plot of the downstream target genes of human miR-22-3p predicted by database DIANA TOOLS, RAID, miRWalk, TargetScan, RNA22, and starBase. b Venn plot of the downstream target genes of mouse miR-22-3p predicted by database miRWalk and DIANA TOOLS. c Venn plot of the downstream genes of both human and mouse miR-22-3p, and the intersection gene is FTO. d DIANA TOOLS website analysis to predict the binding site of the human (top) and mouse (bottom) gene FTO to the 3′UTR region of miR-22-3p (middle). e Analysis of dual-luciferase reporter assay in HEK-293 T cells. f, g The mRNA and protein expression of FTP were analyzed by RT-qPCR and immunoblotting. h, i RT-qPCR and immunoblotting were used to analyze mRNA and protein expression of FTO in mice femoral tissue in each group (n = 12/group) (*p < 0.05 vs. the sham group or the BMSC-EV/inhibitor-NC group or the mimic-NC group, ^#p < 0.05 vs. the inhibitor-NC group or the OVX + BMSC-EV/inhibitor-NC group). Comparison between two groups was performed by unpaired t test. Comparison of data between multiple groups was performed by one-way ANOVA, and Tukey’s was used for post hoc tests