Fig. 5

FTO repression rescues the promotion of osteogenic differentiation by miR-22-3p. BMSC were divided into the BMSC-EV/inhibitor-NC + si-NC, BMSC-EV/inhibitor-NC + si-FTO, BMSC-EV/miR-22-3p inhibitor + si-NC group, and BMSC-EV/miR-22-3p inhibitor + si-FTO groups. a RT-qPCR was adopted to determine the knockdown efficiency of the si-FTO # 1 and si-FTO # 2 groups. b Detection of transfection efficiency by RT-qPCR. c ALP activity of BMSCs was determined using ALP staining (× 400). d ARS staining was adopted to determine the extracellular matrix mineralization of BMSC (× 200). e RT-qPCR was used to detect the mRNA level of RUNX2, OCN, and OPN. f Immunoblotting was adopted to determine the protein level of RUNX2, OCN, and OPN (*p < 0.05 vs. the BMSC-EV/inhibitor-NC + si-NC group, ^#p < 0.05 vs. the BMSC-EV/miR-22-3p inhibitor + si-NC group). Comparison between multiple groups was performed by one-way ANOVA, and Tukey’s was used for post hoc tests